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Image Search Results
Journal:
Article Title: The IRF-3 Transcription Factor Mediates Sendai Virus-Induced Apoptosis
doi:
Figure Lengend Snippet: IFN release is not implicated in IRF-3-induced apoptosis. (A) Control 293 and 293 IRF-3 ΔN-expressing cells were left untreated or were infected with Sendai virus (80 HAU/ml) for 24, 48, and 72 h in the presence or absence of IFN-α (400 IU/ml) or neutralizing antibody for alpha/beta interferon (1/100) (Sigma) as indicated. Viability was measured by using an MTT assay as described in Materials and Methods. Symbols: ■, 293; □, 293 plus IFN-α; ▵, 293 plus anti-IFN-α; ●, 293 IRF-3 ΔN. (B) TUNEL staining of Jurkat cells. The rtTA-Jurkat cells were either left untreated, were infected with Sendai virus (80 HAU/ml), or were treated with IFN-α (400 IU/ml) for 72 h; anti-IFN-α antibody was added with Sendai virus. The number of apoptotic cells was determined by TUNEL as described in Materials and Methods. (C) RPA of IFN-β and IFN-γ mRNA production. The rtTA-, wtIRF-3-, and IRF-3(5D)-expressing 293 and Jurkat cells were cultured in the presence or absence of DOX, as indicated, for 24 h. Cells were then either left untreated or were infected with Sendai virus for 72 h. Total RNA was isolated from each sample and was analyzed by RNase protection analysis by using the human CK-3 RPA kit (Pharmingen), according to manufacturer's instructions.
Article Snippet: The rtTA-, wtIRF-3-, and IRF-3(5D)-expressing 293 and Jurkat cells were cultured in the presence or absence of DOX, as indicated, for 24 h. Cells were then either left untreated or were infected with Sendai virus for 72 h. Total RNA was isolated from each sample and was analyzed by RNase protection analysis by using the
Techniques: Expressing, Infection, MTT Assay, TUNEL Assay, Staining, Cell Culture, Isolation